Regulatory

Part:BBa_K945002:Design

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-26)


CUP1 yeast promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal PstI site found at 49
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal PstI site found at 49
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 55
    Illegal BamHI site found at 510
    Illegal XhoI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal PstI site found at 49
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal PstI site found at 49
    Illegal NgoMIV site found at 36
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 529


Design Notes

The fact that the sequence for this part included restriction sites for XbaI and SpeI poses a problem for its inclusion in an assembly standard.


Source

This sequence was identified in the 5' untranslated region of the metallothionein gene in Saccharomyces cerevisiae.

References